Difference between revisions of "Part:BBa K1088003"
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<partinfo>BBa_K1088003 short</partinfo> | <partinfo>BBa_K1088003 short</partinfo> | ||
− | This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons. | + | This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1) |
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+ | References | ||
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+ | (1) Molecular cloning, expression and characterization of cDNA | ||
+ | encoding cis-prenyltransferases from Hevea brasiliensis | ||
+ | Kasem Asawatreratanakul, Seiji Takahashi, Yuan-Wei Zhang, Atiya Rattanapittayaporn, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul, and Tanetoshi Koyama | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:56, 22 August 2013
Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA
This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)
References
(1) Molecular cloning, expression and characterization of cDNA
encoding cis-prenyltransferases from Hevea brasiliensis
Kasem Asawatreratanakul, Seiji Takahashi, Yuan-Wei Zhang, Atiya Rattanapittayaporn, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul, and Tanetoshi Koyama
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]