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| − | __NOTOC__
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| − | <partinfo>BBa_K1088002 short</partinfo>
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| − | We constructed a system to quantify the protein expression of our desired gene, Dxs, by using the amilCP reporter. The system is under transcriptional control by a sigma 70 dependent promoter and we have used the terminator present in the pSB1C3 plasmid. The Dxs gene is translationally fused to the amilCP gene and therefore the stopcodon of dxs have been removed. The Dxs gene was amplified with primers that carried both RBS and the promoter in its overhang. Also a glycine linker was added between dxs and amilCP.
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K1088002 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K1088002 parameters</partinfo>
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