Difference between revisions of "Part:BBa K1159001:Design"
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<html><table border="1" cellspacing="0" width="100%" !important><tr><td colspan="200" width="20%"><strong>BioBrick: <partinfo>BBa_</partinfo></strong></td><td colspan="800" width="80%"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> version 1.0</strong></td></tr><tr><td colspan="100" width="10%"><strong>RFC standard:</strong></td><td colspan="600" width="60%">RFC 25 BioBrick, so ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.</td><td colspan="300" width="30%">ORF from -8 to 516 (excluding stop-codon)</td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;<u><i>ATGGCCGGC</i>GTGTTCACC&nbsp;...&nbsp;ATCCTTGCT<i>ACCGGT</i></u></span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT<br>101&nbsp;LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG<br></span></td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">4 (2.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">1 (0.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">4 (2.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">3 (1.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">11 (6.3%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">7 (4.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">7 (4.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">18 (10.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">8 (4.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">3 (1.7%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">8 (4.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">8 (4.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">16 (9.1%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">6 (3.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">6 (3.4%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">12 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">22 (12.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">7 (4.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">6 (3.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">18 (10.3%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number of amino acids (aa):</td><td colspan="100" width="10.0%">175</td><td colspan="200" width="20.0%">Positively charged aa (Arg + Lys):</td><td colspan="100" width="10.0%">14</td><td colspan="200" width="20.0%">Negatively charged aa (Asp + Glu):</td><td colspan="100" width="10.0%">20</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular weight [Da]:</td><td colspan="100" width="10.0%">19381.3</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.1</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">25502.5 (all Cys as cystine)<br>25440 (all Cys reduced)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life [h]:</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">>20</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">30</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage (CAI):</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">0.66</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">0.62</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">0.82</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br></html>
  
 
===Source===
 
===Source===

Revision as of 18:26, 27 July 2013

NanoLuc Luciferase in RFC[25]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:



BioBrick: BBa_Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0
RFC standard:RFC 25 BioBrick, so ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.ORF from -8 to 516 (excluding stop-codon)
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 ATGGCCGGCGTGTTCACC ... ATCCTTGCTACCGGT
Amino acid sequence:
  1 MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT
101 LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG
Amino acid composition:
A (Ala)4 (2.3%)C (Cys)1 (0.6%)H (His)4 (2.3%)M (Met)3 (1.7%)T (Thr)11 (6.3%)
R (Arg)7 (4.0%)Q (Gln)7 (4.0%)I (Ile)18 (10.3%)F (Phe)8 (4.6%)W (Trp)3 (1.7%)
N (Asn)8 (4.6%)E (Glu)8 (4.6%)L (Leu)16 (9.1%)P (Pro)6 (3.4%)Y (Tyr)6 (3.4%)
D (Asp)12 (6.9%)G (Gly)22 (12.6%)K (Lys)7 (4.0%)S (Ser)6 (3.4%)V (Val)18 (10.3%)
Amino acid counting:Total number of amino acids (aa):175Positively charged aa (Arg + Lys):14Negatively charged aa (Asp + Glu):20
Biochemical parameters:Molecular weight [Da]:19381.3Theoretical pI:5.1Extinction coefficient at 280 nm [M-1 cm-1]:25502.5 (all Cys as cystine)
25440 (all Cys reduced)
Estimated half-life [h]:E. coli:>10S. cervisiae:>20Mammals:30
Codon usage (CAI):E. coli:0.66S. cervisiae:0.62Mammals:0.82
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.

Source

Source:


Organism:


References

Literature references:

Database references: