Difference between revisions of "Part:BBa K1159003"

Revision as of 16:06, 27 July 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

Orange	Apple	12,333.00
Bread	Pie	500.00
Butter	Ice cream	1.00

Automatically determined parameters using the BioBrick-AutoAnnotator Version 1.0

Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
GTACACAATGCGTCGT ... TTCGAAAAATAA

Amino acid sequence:
1 MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
101 FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
201 FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
301 DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
401 ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
501 FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK

BioBrick: BBa_K801060

Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).

Amino acid composition:

A (Ala)

33 (5.8%)

C (Cys)

4 (0.7%)

H (His)

18 (3.2%)

M (Met)

16 (2.8%)

T (Thr)

26 (4.6%)

R (Arg)

25 (4.4%)

Q (Gln)

24 (4.2%)

I (Ile)

39 (6.9%)

F (Phe)

28 (4.9%)

W (Trp)

14 (2.5%)

N (Asn)

27 (4.8%)

E (Glu)

48 (8.5%)

L (Leu)

64 (11.3%)

P (Pro)

17 (3.0%)

Y (Tyr)

27 (4.8%)

D (Asp)

34 (6.0%)

G (Gly)

29 (5.1%)

K (Lys)

36 (6.4%)

S (Ser)

33 (5.8%)

V (Val)

24 (4.2%)

Amino acid counting:

Total number of amino acids (aa):

566

Number of positively charged aa (Arg + Lys):

61

Number of negatively charged aa (Asp + Glu):

82

Biochemical parameters:

Molecular weight [Da]:

66105.33

Theoretical pI:

5.38

Extinction coefficient at 280 nm [M^-1 cm^-1]:

117480 (all Cys as cystine)
117230 (all Cys reduced)

Estimated half-life:

Mammals:

30 h

Yeast:

>20 h

E. coli:

>10 h

Codon usage: (CAI)

Mammals:

0.68

Yeast:

0.69

E. coli:

0.71

RFC standard:

This is a RFC 10 BioBrick, nothing was added

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 |12,333.00