Difference between revisions of "Part:BBa K1159003"
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<table border="1" cellspacing="0" width="100%" !important><tr><td colspan="1000"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> Version 1.0</strong></td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;GTACACA<u>ATGCGTCGT&nbsp;...&nbsp;TTCGAAAAA</u>TAA</span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>101&nbsp;FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>201&nbsp;FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>301&nbsp;DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>401&nbsp;ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>501&nbsp;FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK<br></span></td></tr><tr><td colspan="200" width="20%"><strong>BioBrick: &lt;partinfo&gt;BBa_K801060&lt;/partinfo&gt;</strong></td><td colspan="800" width="80%">Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).</td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">4 (0.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">18 (3.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">16 (2.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">26 (4.6%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">25 (4.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">24 (4.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">39 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">28 (4.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">14 (2.5%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">27 (4.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">48 (8.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">64 (11.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">17 (3.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">27 (4.8%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">34 (6.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">29 (5.1%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">36 (6.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">24 (4.2%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number of amino acids (aa):</td><td colspan="100" width="10.0%">566</td><td colspan="200" width="20.0%">Number of positively charged aa (Arg + Lys):</td><td colspan="100" width="10.0%">61</td><td colspan="200" width="20.0%">Number of negatively charged aa (Asp + Glu):</td><td colspan="100" width="10.0%">82</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular weight [Da]:</td><td colspan="100" width="10.0%">66105.33</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.38</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">117480 (all Cys as cystine)<br>117230 (all Cys reduced)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life:</strong></td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">30 h</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">>20 h</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10 h</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage:</strong> (CAI)</td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">0.68</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">0.69</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">0.71</td></tr><tr><td colspan="200" width="20.0%"><strong>RFC standard:</strong></td><td colspan="800" width="80.0%">This is a RFC 10 BioBrick, nothing was added</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table>
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<table border="1" cellspacing="0" width="100%" !important><tr><td colspan="1000"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> Version 1.0</strong></td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;GTACACA<u>ATGCGTCGT&nbsp;...&nbsp;TTCGAAAAA</u>TAA</span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>101&nbsp;FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>201&nbsp;FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>301&nbsp;DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>401&nbsp;ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>501&nbsp;FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK<br></span></td></tr><tr><td colspan="200" width="20%"><strong>BioBrick: <partinfo>BBa_K801060</partinfo></strong></td><td colspan="800" width="80%">Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).</td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">4 (0.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">18 (3.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">16 (2.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">26 (4.6%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">25 (4.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">24 (4.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">39 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">28 (4.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">14 (2.5%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">27 (4.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">48 (8.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">64 (11.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">17 (3.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">27 (4.8%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">34 (6.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">29 (5.1%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">36 (6.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">24 (4.2%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number of amino acids (aa):</td><td colspan="100" width="10.0%">566</td><td colspan="200" width="20.0%">Number of positively charged aa (Arg + Lys):</td><td colspan="100" width="10.0%">61</td><td colspan="200" width="20.0%">Number of negatively charged aa (Asp + Glu):</td><td colspan="100" width="10.0%">82</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular weight [Da]:</td><td colspan="100" width="10.0%">66105.33</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.38</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">117480 (all Cys as cystine)<br>117230 (all Cys reduced)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life:</strong></td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">30 h</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">>20 h</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10 h</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage:</strong> (CAI)</td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">0.68</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">0.69</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">0.71</td></tr><tr><td colspan="200" width="20.0%"><strong>RFC standard:</strong></td><td colspan="800" width="80.0%">This is a RFC 10 BioBrick, nothing was added</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table>
 
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Revision as of 15:38, 27 July 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

Automatically determined parameters using the BioBrick-AutoAnnotator Version 1.0
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 GTACACAATGCGTCGT ... TTCGAAAAATAA
Amino acid sequence:
  1 MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
101 FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
201 FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
301 DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
401 ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
501 FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK
BioBrick: BBa_K801060Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).
Amino acid composition:
A (Ala)33 (5.8%)C (Cys)4 (0.7%)H (His)18 (3.2%)M (Met)16 (2.8%)T (Thr)26 (4.6%)
R (Arg)25 (4.4%)Q (Gln)24 (4.2%)I (Ile)39 (6.9%)F (Phe)28 (4.9%)W (Trp)14 (2.5%)
N (Asn)27 (4.8%)E (Glu)48 (8.5%)L (Leu)64 (11.3%)P (Pro)17 (3.0%)Y (Tyr)27 (4.8%)
D (Asp)34 (6.0%)G (Gly)29 (5.1%)K (Lys)36 (6.4%)S (Ser)33 (5.8%)V (Val)24 (4.2%)
Amino acid counting:Total number of amino acids (aa):566Number of positively charged aa (Arg + Lys):61Number of negatively charged aa (Asp + Glu):82
Biochemical parameters:Molecular weight [Da]:66105.33Theoretical pI:5.38Extinction coefficient at 280 nm [M-1 cm-1]:117480 (all Cys as cystine)
117230 (all Cys reduced)
Estimated half-life:Mammals:30 hYeast:>20 hE. coli:>10 h
Codon usage: (CAI)Mammals:0.68Yeast:0.69E. coli:0.71
RFC standard:This is a RFC 10 BioBrick, nothing was added
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]