Difference between revisions of "Part:BBa M1001"

Latest revision as of 04:19, 21 June 2006

Right mosaic end for Tn5 transpososome

Right end of a linear transposome sequence containing the 19 bp mosaic end in the reverse orientation. The sequence also contains a PvuII cut site which releases the transposome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.

This part, and the companion part M1000 provide Biobrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome.
The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_M1001 short</partinfo>
-Right end of a linear transpososome sequence containing the 19 bp mosaic end in the reverse orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.
+*Right end of a linear transposome sequence containing the 19 bp mosaic end in the reverse orientation. The sequence also contains a PvuII cut site which releases the transposome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.
-This part, and the companion part M1000 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome.
+*This part, and the companion part M1000 provide Biobrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome.
-The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other  functional chromosomal insertions.
+*The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other  functional chromosomal insertions.