Difference between revisions of "Part:BBa K1159015:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1159015 short</partinfo>
 
<partinfo>BBa_K1159015 short</partinfo>
Line 5: Line 4:
 
<partinfo>BBa_K1159015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1159015 SequenceAndFeatures</partinfo>
  
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 +
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
 +
 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 +
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
−
x
 
  
 +
'''Related BioBrick:'''
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 +
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
 +
'''Cloning details:'''<br>
 +
<!--*Designed in RFC10/RFC23/RFC25-->
 +
<!--*Mutation C889G to delete XbaI restriction site-->
 +
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
 +
'''Quality control measures:'''<br>
 +
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 +
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 +
<!--*Part was partly sequenced/Part was totally sequenced-->
 +
 +
'''Backbone:'''<br>
 +
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 +
<!--*Resistance: Amp/Cp/Kan/-->
 +
<!--*Copynumber: low/medium/high-->
 +
 +
'''Protein coding:'''<br>
 +
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
 +
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 +
<!--*The protein encoded is posttranslationally modified by ???.-->
 +
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 +
 +
'''Enzymatic activity:'''
 +
<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br>
 +
<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
 +
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
x
+
'''Source:'''<br>
 +
<!--*Commercial system: plasmid name, system name, company name-->
 +
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 +
<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
 +
 
 +
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 
 +
'''Organism:'''<br>
 +
<!--*Genesequence derived from ''?organism_name?''-->
 +
<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
 +
 
 +
<!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []-->
 +
 
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 14:54, 17 May 2013

Membrane-anchored NanoLuc Luciferase in RFC[25] N-Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 83
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 597
    Illegal BsaI.rc site found at 1515


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: