Difference between revisions of "Part:BBa K511825"
(rollbackEdits.php mass rollback)
 
Line 1: Line 1:
−
1005123605 http://manualip2cj.pp.ua/vnauw2.html http://documentspacs.pp.ua/ http://manualip2cj.pp.ua/ies1.html http://documents140.pp.ua/coyx1.html http://documents4xm7.pp.ua/ http://documentsjgq.pp.ua/  http://manualiguj.pp.ua/ http://instructionsqsa.pp.ua/cfco1.html    http://instructionsqsa.pp.ua/kml2.html  http://documentsjgq.pp.ua/bmy2.html        http://documents140.pp.ua/rlivwk2.html    http://manualiguj.pp.ua/tfhm1.html  http://documents140.pp.ua/          http://documentsjgq.pp.ua/kuelta1.html http://manualip2cj.pp.ua/    http://manualiguj.pp.ua/evr2.html
+
 
 +
__NOTOC__
 +
<partinfo>BBa_K511825 short</partinfo>
 +
 
 +
This MammoBlock composite device produces the Notch-Gal4-ESN juxtacrine signaling receptor when induced with Gal4 transactivator variants and otherwise produces Notch-Gal4-ESN at a low, basal (OFF) level. This device is applicable to cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact. Note that in this particular case, activation of Notch-Gal4-ESN will release the same transactivator necessary for production of Notch-Gal4-ESN, creating a positive feedback loop upon activation.
 +
 
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K511825 SequenceAndFeatures</partinfo>
 +
 
 +
 
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K511825 parameters</partinfo>
 +
<!-- -->

Latest revision as of 16:18, 10 May 2013


Inducible Notch-Gal4-ESN Receptor Generator (UAS-Gal4-Notch-Gal4-ESN) MammoBlock Device

This MammoBlock composite device produces the Notch-Gal4-ESN juxtacrine signaling receptor when induced with Gal4 transactivator variants and otherwise produces Notch-Gal4-ESN at a low, basal (OFF) level. This device is applicable to cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact. Note that in this particular case, activation of Notch-Gal4-ESN will release the same transactivator necessary for production of Notch-Gal4-ESN, creating a positive feedback loop upon activation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
    Illegal NotI site found at 5458
    Illegal NotI site found at 5466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal BamHI site found at 3167
    Illegal XhoI site found at 83
    Illegal XhoI site found at 5749
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 2870
    Illegal NgoMIV site found at 2926
    Illegal NgoMIV site found at 2969
    Illegal NgoMIV site found at 3754
    Illegal NgoMIV site found at 4771
    Illegal NgoMIV site found at 4948
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5668
    Illegal BsaI.rc site found at 327
    Illegal BsaI.rc site found at 2035
    Illegal BsaI.rc site found at 2950
    Illegal BsaI.rc site found at 5407