Difference between revisions of "Part:BBa K511823"

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__NOTOC__
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<partinfo>BBa_K511823 short</partinfo>
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This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level.
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<html><img src='https://static.igem.org/mediawiki/parts/e/ee/UAS_data.jpg' style="width:60%"><br></html>
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Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels.
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As can be clearly seen, Hef1a:GV16 results in significant activation of the UAS promoter. This results in the observed increases in mean fluoresence compared to control populations. With the exception of UAS:eBFP2, we see more than 20-fold increase in mean fluorescence.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K511823 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K511823 parameters</partinfo>
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Revision as of 11:15, 6 May 2013

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