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| − | __NOTOC__
| + | 0605 http://manualss7iy.pp.ua/bnwrwn1.html http://manualss7iy.pp.ua/xpem2.html http://documentsoex.pp.ua/tsq1.html http://documentsoex.pp.ua/kvxtcu2.html http://instructions9rxg.pp.ua/iiejn2.html http://instructions9rxg.pp.ua/lddpim4.html http://instruktsiya721.pp.ua/olkkpx2.html http://instruktsiya721.pp.ua/vrol1.html http://rukovodstnnr.pp.ua/mwcfk2.html http://rukovodstnnr.pp.ua/xuykyh3.html http://rukovodstnnr.pp.ua/juqpxs2.html http://rukovodstnnr.pp.ua/wil3.html http://instruktsiya5nd.pp.ua/mvcgo2.html http://instruktsiya5nd.pp.ua/ytjmtg3.html http://rukovodstvqk.pp.ua/tqu2.html http://rukovodstvqk.pp.ua/kjanan3.html |
| − | <partinfo>BBa_K511301 short</partinfo>
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| − | This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with the Gal4-VP16 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.
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| − | This version of the Notch signaling protein is expected to induce much stronger levels of activation than those documented in recent work by Sprinzak, et al.
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| − | For more information, consult the MIT iGEM 2011 wiki <html><a href="http://2011.igem.org/Team:MIT/Results">here.</a></html>
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| − | Sprinzak, D. et al. Cis interactions between Notch and Delta generate mutually exclusive signaling states. Nature 25 April 2010
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| − | <html><a href="http://www.nature.com/nature/journal/v465/n7294/full/nature08959.html">http://www.nature.com/nature/journal/v465/n7294/full/nature08959.html</a></html>
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K511301 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K511301 parameters</partinfo>
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Revision as of 11:15, 6 May 2013
0605 http://manualss7iy.pp.ua/bnwrwn1.html http://manualss7iy.pp.ua/xpem2.html http://documentsoex.pp.ua/tsq1.html http://documentsoex.pp.ua/kvxtcu2.html http://instructions9rxg.pp.ua/iiejn2.html http://instructions9rxg.pp.ua/lddpim4.html http://instruktsiya721.pp.ua/olkkpx2.html http://instruktsiya721.pp.ua/vrol1.html http://rukovodstnnr.pp.ua/mwcfk2.html http://rukovodstnnr.pp.ua/xuykyh3.html http://rukovodstnnr.pp.ua/juqpxs2.html http://rukovodstnnr.pp.ua/wil3.html http://instruktsiya5nd.pp.ua/mvcgo2.html http://instruktsiya5nd.pp.ua/ytjmtg3.html http://rukovodstvqk.pp.ua/tqu2.html http://rukovodstvqk.pp.ua/kjanan3.html
