Difference between revisions of "Part:BBa K646000"

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__NOTOC__
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<partinfo>BBa_K646000 short</partinfo>
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Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
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===Usage and Biology===
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[[Image:MQ_New_data_page_diagram.jpg|center|750px]] <br> '''The BioBrick part in team Macquarie University (2011) mechanism'''<br><br>
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When coupled to a promoter BioBrick such as T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]) to produce parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646003 BBa_K646003] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646004 BBa_K646004], and transformed into Bl21(DE3)Tuner ''E. coli'' cells, a green colouration (require induction with ALA and IPTG) is produced.
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[[Image:Results_-_green colonies.JPG|400px]]
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Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green due to the production of biliverdin from the breakdown of heme - this indicates that heme oxyganse was expressed using T7 promoter.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K646000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K646000 parameters</partinfo>
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Revision as of 11:15, 6 May 2013

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