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| − | __NOTOC__
| + | 0605 http://manualss7iy.pp.ua/bnwrwn1.html http://manualss7iy.pp.ua/xpem2.html http://documentsoex.pp.ua/tsq1.html http://documentsoex.pp.ua/kvxtcu2.html http://instructions9rxg.pp.ua/iiejn2.html http://instructions9rxg.pp.ua/lddpim4.html http://instruktsiya721.pp.ua/olkkpx2.html http://instruktsiya721.pp.ua/vrol1.html http://rukovodstnnr.pp.ua/mwcfk2.html http://rukovodstnnr.pp.ua/xuykyh3.html http://rukovodstnnr.pp.ua/juqpxs2.html http://rukovodstnnr.pp.ua/wil3.html http://instruktsiya5nd.pp.ua/mvcgo2.html http://instruktsiya5nd.pp.ua/ytjmtg3.html http://rukovodstvqk.pp.ua/tqu2.html http://rukovodstvqk.pp.ua/kjanan3.html |
| − | <partinfo>BBa_K511302 short</partinfo>
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| − | This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein.
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| − | In complex eukaryotic systems, Delta is a single-pass transmembrane protein that functions as a ligand for another single-pass receptor known as Notch. Binding of Delta to Notch results in cleavage of the intracellular domain of Notch by a ubiquitously expressed protease. The cleaved intracellular domain is then translocated to the nucleus of the Notch-bearing cell and is able to activate transcription of target genes. If Delta and Notch are expressed on the same cell, however, Delta binds to a second location on the Notch receptor, forming a complex that cannot be activated by Delta from another cell.
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| − | The C-terminal fusion of the mCherry protein to this signaling ligand means this protein is also a useful marker for transfection identification and/or membrane colocalization experiments.
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <!-- -->
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K511302 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K511302 parameters</partinfo>
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| − | <!-- -->
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| − | ==Characterization==
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| − | [[Image:NotchDeltaActivation.png|thumb|left|Figure 1. Activation of Notch-Gal4-ESN by Delta-mCherry in a HEK-293 Sender - CHO Receiver Co-Culture.]]
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| − | Figure 1 to the left shows activation of chinese hamster ovary (CHO) cells bearing Notch-Gal4-ESN driven by the CMV promoter and Citrine driven by the UAS-Gal4 promoter by human embryonic kidney (HEK-293) cells bearing Delta-mCherry driven by the Hef1a-LacO promoter. The images is an overlay of yellow fluorescence from Citrine and red fluorescence from Delta-mCherry. The abnormal morphology of red-fluorescence here was likely due to bleed-through, thus making cells appear larger than they are.
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| − | <html><br><br><br><br><br><br><br><br><br><br>
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| − | <center><b>Timelapse of CHO cells transfected with CMV:tTA and TRE:Delta-mCherry with dox<br>
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| − | <iframe width="420" height="315" src="http://www.youtube.com/embed/oHJhDiTZZCI" frameborder="0" allowfullscreen></iframe></center>
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| − | </b><br>
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| − | The video is a timelapse of CHO cells transfected with CMV:tTA and TRE:Delta-mCherry. At t=0, 100ug/mL dox is added to the media. The movie is a timelapse sped up such that one second of movie time is one hour of real time.
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| − | </html>
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Revision as of 11:15, 6 May 2013
0605 http://manualss7iy.pp.ua/bnwrwn1.html http://manualss7iy.pp.ua/xpem2.html http://documentsoex.pp.ua/tsq1.html http://documentsoex.pp.ua/kvxtcu2.html http://instructions9rxg.pp.ua/iiejn2.html http://instructions9rxg.pp.ua/lddpim4.html http://instruktsiya721.pp.ua/olkkpx2.html http://instruktsiya721.pp.ua/vrol1.html http://rukovodstnnr.pp.ua/mwcfk2.html http://rukovodstnnr.pp.ua/xuykyh3.html http://rukovodstnnr.pp.ua/juqpxs2.html http://rukovodstnnr.pp.ua/wil3.html http://instruktsiya5nd.pp.ua/mvcgo2.html http://instruktsiya5nd.pp.ua/ytjmtg3.html http://rukovodstvqk.pp.ua/tqu2.html http://rukovodstvqk.pp.ua/kjanan3.html
