Difference between revisions of "Part:BBa K427004"
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__NOTOC__
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<partinfo>BBa_K427004 short</partinfo>
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Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
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===Usage and Biology===
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The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins.
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This part has no special safety considerations.
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===<span class='h3bb'>Sequence and Features</span>===
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<partinfo>BBa_K427004 SequenceAndFeatures</partinfo>
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===References===
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Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K427004 parameters</partinfo>
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Revision as of 11:15, 6 May 2013

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