Difference between revisions of "Part:BBa M36099:Design"
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===Design Notes=== | ===Design Notes=== | ||
Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0. | Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0. | ||
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| + | rpo_bla and rrnB1T1T2 transcription terminators serve as insulators. | ||
===Source=== | ===Source=== | ||
Revision as of 21:13, 23 April 2013
E. coli sensor test rig
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188
Illegal AgeI site found at 2605 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0.
rpo_bla and rrnB1T1T2 transcription terminators serve as insulators.
Source
Gemini coding sequence sourced from Dr. Drew Endy and Martin et al., PlosOne 2009. The rest of the vector is sourced from DNA2.0.