Difference between revisions of "Part:pSB1T3:Design"

Revision as of 18:51, 5 February 2013

High copy BioBrick assembly plasmid

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2440
Illegal NheI site found at 1268
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2446
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2440
Illegal BamHI site found at 1414
Illegal XhoI site found at 1033
Illegal XhoI site found at 2316
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2440
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2440
Plasmid lacks a suffix.
Illegal XbaI site found at 2455
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1440
Illegal NgoMIV site found at 1808
Illegal NgoMIV site found at 1968
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 8: / Line 8: @@
 The ampicillin resistance marker needed to be eliminated.
-In conversation with Austin Che, I gather this plasmid was made by taking a plasmid that was probably pSB1A3 and replacing the antibiotic resistance marker and sequence between two XhoI sites with a PCR product that encoded the Tetracycline resistance marker and could be digested with XhoI.  This was done in a non-directional manner.  Sequence data for a plasmid derived from this plasmid suggests that the Tet marker was cloned in the reverse orientation to that shown here.  Sequencing of the Registry clone would likely confirm this, at which point the sequence of this molecule should be changed.
 ===Source===