Difference between revisions of "Help:Plasmid backbones/Features"
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==Origin (of replication)== | ==Origin (of replication)== | ||
The origin of replication is a binding sequence where replication proteins which nick, unwind, and replicate plasmid DNA independently of the cellular host. | The origin of replication is a binding sequence where replication proteins which nick, unwind, and replicate plasmid DNA independently of the cellular host. | ||
| − | Many biobrick plasmids have the following | + | Many biobrick plasmids have the following type of origin of replication: |
| − | *pmB1 - This origin has been isolated from one of the first plasmids taken from a human body. It is a "relaxed" plasmid, meaning that it allows high copy amounts within a cell | + | *<b>pmB1</b> - This origin has been isolated from one of the first plasmids taken from a human body. It is a "relaxed" plasmid, meaning that it allows high copy amounts within a cell |
| − | + | ||
| − | This origin is not to be confused with the "origin of ''transfer''", the site used in conjugal rolling circle replication transfer. | + | Note: This origin is not to be confused with the "origin of ''transfer''", the site used in conjugal rolling circle replication transfer. |
==Links and References== | ==Links and References== | ||
*[http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html "Cloning Vectors and Genetic Engineering"] | *[http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html "Cloning Vectors and Genetic Engineering"] | ||
Revision as of 20:24, 18 June 2006
- Information on what the feature tables of plasmids mean
Copy Number
Most Biobricks are "high copy" type plasmids and are well-suited for high yield plasmid purification. These plasmids can reach large numbers by being replicated using host enzymes (source: [http://www.biochem.wisc.edu/courses/biochem651_f05/Lectures/08Plasmids/Theory_plasmids/copynumber.html University of Wisc. Madison])
Large DNA inserts can greatly reduce the copy number of a plasmid, despite the fact that they may originally be high-copy.
Origin (of replication)
The origin of replication is a binding sequence where replication proteins which nick, unwind, and replicate plasmid DNA independently of the cellular host. Many biobrick plasmids have the following type of origin of replication:
- pmB1 - This origin has been isolated from one of the first plasmids taken from a human body. It is a "relaxed" plasmid, meaning that it allows high copy amounts within a cell
Note: This origin is not to be confused with the "origin of transfer", the site used in conjugal rolling circle replication transfer.
Links and References
- [http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html "Cloning Vectors and Genetic Engineering"]