Difference between revisions of "Part:BBa K747102:Design"

(→‎Source)
(→‎Source)
Line 13: Line 13:
  
 
The TAL-protein was build with the following parts:
 
The TAL-protein was build with the following parts:
 +
 
BBa_K747009: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K747009 GC]
 
BBa_K747009: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K747009 GC]
  

Revision as of 10:01, 31 October 2012

pTAL-AID


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3200
    Illegal XbaI site found at 3253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1999
    Illegal SpeI site found at 3201
    Illegal PstI site found at 3215
    Illegal NotI site found at 3208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3241
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3201
    Illegal XbaI site found at 3253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3253
    Illegal SpeI site found at 3201
    Illegal PstI site found at 3215
    Illegal NgoMIV site found at 3823
    Illegal NgoMIV site found at 5106
    Illegal NgoMIV site found at 5389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2071
    Illegal BsaI.rc site found at 1417
    Illegal BsaI.rc site found at 2233
    Illegal BsaI.rc site found at 2722
    Illegal BsaI.rc site found at 5601
    Illegal SapI site found at 2823
    Illegal SapI.rc site found at 4955
    Illegal SapI.rc site found at 5165


Design Notes

see main page

Source

see for further information: Eukaryotic TAL expression plasmid

The TAL-protein was build with the following parts:

BBa_K747009: GC

BBa_K747026:GG

BBa_K747045:TC

BBa_K747060:TA

BBa_K747079:TT

BBa_K747081:AC

The AID was constructed by the IGEM 2012 team of Potsdam: modified AID

References

1. Scholze, H. & Boch, J. TAL effectors are remote controls for gene activation. Current Opinion in Microbiology 14, 47–53 (2011).

2. Moscou, M. J. & Bogdanove, A. J. A Simple Cipher Governs DNA Recognition by TAL Effectors. Science 326, 1501–1501 (2009).

3. Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 39, e82 (2011).

4. Reyon, D. et al. FLASH assembly of TALENs for high-throughput genome editing. Nature Biotechnology 30, 460–465 (2012).

5. Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nature biotechnology 29, 149–153 (2011).

6. Miller, J. C. et al. A TALE nuclease architecture for efficient genome editing. Nature Biotechnology 29, 143–148 (2010).

7. Boch, J. et al. Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors. Science 326, 1509–1512 (2009).

8. Liu, J. et al. Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy. Journal of Genetics and Genomics 39, 209–215 (2012).

9. Wood, A. J. et al. Targeted Genome Editing Across Species Using ZFNs and TALENs. Science 333, 307–307 (2011).

10. Sander, J. D. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29, 697–698 (2011).

11. Tesson, L. et al. Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology 29, 695–696 (2011).

12. Hockemeyer, D. et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nature Biotechnology 29, 731–734 (2011) 13. Sanjana, N. E. et al. A transcription activator-like effector toolbox for genome engineering. Nature Protocols 7, 171–192 (2012).