Difference between revisions of "Part:BBa K917009:Experience"

Revision as of 11:38, 30 October 2012

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Applications of BBa_K917009

We have tested the expression of CymA proteins in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and cymA gene). Cell pellets were scanned prior to sonication and cymA transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.

Haem staining results suggest cymA expression in E coli transformed with plac-cymA and plac-ccm. The additional band indicated by an arrow in lane 3 represents cymA protein.

User Reviews

UNIQc8948dc4fcbec9b1-partinfo-00000000-QINU UNIQc8948dc4fcbec9b1-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K917009===
+We have tested the expression of CymA proteins in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and cymA gene). Cell pellets were scanned prior to sonication and cymA transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.
+[[Image:Cytochrome compare.jpg]]
+Haem staining results suggest cymA expression in E coli transformed with plac-cymA and plac-ccm. The additional band indicated by an arrow in lane 3 represents cymA protein.
+[[Image:Gel_haem_compare_.jpg]]
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K917009 StartReviews</partinfo>