Difference between revisions of "Part:BBa K775000"
Line 19: Line 19:
 
<html>
 
<html>
 
<body>
 
<body>
−
<img src="https://static.igem.org/mediawiki/2012/1/15/NR1_immuno.png" size="70%"><br>
+
<img src="https://static.igem.org/mediawiki/2012/1/15/NR1_immuno.png" width="300"><br>
 
<p>Immunolocalization of the receptor protein using FLAG treatment. A halo formation indicates appropriate localization in the membrane </p><br><br/>
 
<p>Immunolocalization of the receptor protein using FLAG treatment. A halo formation indicates appropriate localization in the membrane </p><br><br/>
−
<img src="https://static.igem.org/mediawiki/igem.org/0/0d/DNAstainingNR1.jpg"><br>
+
<img src="https://static.igem.org/mediawiki/igem.org/0/0d/DNAstainingNR1.jpg" width="300"><br>
 
<p>Flow cytometry results of cells stained with Vybrant® DyeCycle™ Orange DNA Stain after 4.40 hours after activation with a saturative amount of nicotinic acid. In the middle and right picture a shift of peaks can be observed, corresponding with formation of more haploid cells (left) in comparison with diploid cells (right).
 
<p>Flow cytometry results of cells stained with Vybrant® DyeCycle™ Orange DNA Stain after 4.40 hours after activation with a saturative amount of nicotinic acid. In the middle and right picture a shift of peaks can be observed, corresponding with formation of more haploid cells (left) in comparison with diploid cells (right).
 
</p>
 
</p>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 20:50, 29 October 2012

RI7-GPR109A Niacin chimeric GPCR

BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 478



Immunolocalization of the receptor protein using FLAG treatment. A halo formation indicates appropriate localization in the membrane




Flow cytometry results of cells stained with Vybrant® DyeCycle™ Orange DNA Stain after 4.40 hours after activation with a saturative amount of nicotinic acid. In the middle and right picture a shift of peaks can be observed, corresponding with formation of more haploid cells (left) in comparison with diploid cells (right).