Difference between revisions of "Part:BBa K792001"

Latest revision as of 03:18, 29 October 2012

Kozak sequence from yeast α-factor mating pheromone (MFα1)

The Kozak sequence is the eukaryotic analog of the bacterial RBS, it is short sequence that includes the ATG, required for proficient initiation of translation. It is known that the 5’UTR sequence of the MFα1 gene of yeast produces efficient initiation of translation.

This part is that specific region of the MFα1 gene.

See also:

A yeast secretion tag from α-factor mating pheromone (MFα1) BBa_K792002

This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 '''This part is that specific region of the MFα1 gene'''.
+{| style="width:100%"
+|-
+| style="background: yellow; width:11%" rowspan="2"| '''See also: '''
+| style="background: #D8D8D8" | A yeast secretion tag from α-factor mating pheromone (MFα1) <partinfo>K792002</partinfo>
+|}
+{| style="width:100%; background: #CEE3F6"
+|-
+|This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a ''cross-feeding'' regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.
+|[[Image:Igem.bsas.png | 100px]]
+|}
 <!-- Add more about the biology of this part here