Difference between revisions of "Part:BBa K902068"

Revision as of 20:36, 28 October 2012

RhaS

RhaS is a transcription factor which is part of the rhaSR operon.

Usage and Biology

RhaS activates expression of the rhaBAD operon on the P_rha rhamnose promoter. In order for it to bind to P_rha, the CRP-cAMP (cAMP receptor protein) complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

The [2012.igem.org/Team:Calgary iGEM Calgary 2012 team] intended to upregulate rhaS so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 440
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 172
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-This serves as regulatory element which activates expression of the pBAD operon on the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902065 pRha rhamnose promoter]. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
+RhaS activates expression of the <i>rhaBAD</i> operon on the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902065 P<sub>rha</sub> rhamnose promoter]. In order for it to bind to P<sub>rha</sub>, the CRP-cAMP (cAMP receptor protein) complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
-In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR]-rhamnose complex (Egan & Schlief, 1993).
+In its native E. coli, the <i>rhaS</i> (on the <i>rhaSR</i> operon off the pRha promoter) is upregulated by the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR]-rhamnose complex (Egan & Schlief, 1993).
-Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
+The [2012.igem.org/Team:Calgary iGEM Calgary 2012 team] intended to upregulate <i>rhaS</i> so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.