Difference between revisions of "Part:BBa K902068"
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<partinfo>BBa_K902068 short</partinfo>
 
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RhaS is a regulatory protein which is part of the RhaSR operon. This is the transcription factor that binds to the pRha promoter and upregulates the rhamnose system in the presence of rhamnose.  
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RhaS is a transcription factor which is part of the <i>rhaSR</i> operon.
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===Usage and Biology===
 
===Usage and Biology===
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This serves as regulatory element which activates expression of the pBAD operon on the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902065 pRha rhamnose promoter]. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
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In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR]-rhamnose complex (Egan & Schlief, 1993).
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Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
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Revision as of 20:30, 28 October 2012

RhaS

RhaS is a transcription factor which is part of the rhaSR operon.

Usage and Biology

This serves as regulatory element which activates expression of the pBAD operon on the pRha rhamnose promoter. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]