Difference between revisions of "Part:BBa K902068:Design"

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===Design Notes===
 
===Design Notes===
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This serves as regulatory element which activates expression of the pBAD operon on the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902065 pRha rhamnose promoter]. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
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This part was inserted into pSB1C3 using Biobrick Assembly Standards.
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In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR]-rhamnose complex (Egan & Schlief, 1993).
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Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
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===Source===
 
===Source===

Latest revision as of 20:28, 28 October 2012

RhaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was inserted into pSB1C3 using Biobrick Assembly Standards.

Source

PCR amplified from Top Ten E. coli with Kapa polymerase.

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.