Difference between revisions of "Part:BBa K902069"

Revision as of 20:25, 28 October 2012

RhaR

RhaR is a regulatory element for the rhamnose metabolic genes in E. coli. In the presence of rhamnose, it complexes with the sugar to upregulate the RhaSR operon. RhaS is produced which in turn binds to the P_rha promoter to upregulate the RhaBAD operon (Egan & Schleif, 1993).

Note that this upregulation is dependent on CRP-cAMP (cAMP receptor protein) being bound to the promoter. In the presence of glucose, cAMP levels are low and this rhamnose cascade is repressed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 559
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K902069 short</partinfo>
-<html><p>RhaR is a regulatory element of the rhamnose system. In the presence of rhamnose it complexes with the sugar and upregulates the RhaSR operon.This in turn upregulates production of RhaS which then binds to the pRha promoter and upregulates the system dramatically. </p></html>
+RhaR is a regulatory element for the rhamnose metabolic genes in <i>E. coli</i>. In the presence of rhamnose, it complexes with the sugar to upregulate the RhaSR operon. RhaS is produced which in turn binds to the P<sub>rha</sub> promoter to upregulate the RhaBAD operon (Egan & Schleif, 1993).
+Note that this upregulation is dependent on CRP-cAMP (cAMP receptor protein) being bound to the promoter. In the presence of glucose, cAMP levels are low and this rhamnose cascade is repressed.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===