Difference between revisions of "Part:BBa K902069:Design"

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===Design Notes===
 
===Design Notes===
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RhaR is present at basal levels in cells. It binds with the inducer rhamnose to upregulate the rhaSR operon off the pRha rhamnose promoter. In turn, more RhaR and RhaS is produced, where RhaS can then activate the rhaBAD operon on the opposite side of the rhamnose promoter (Egan & Schleif, 1993).
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This part was added to pSB1C3 using Biobrick Assembly Standards.
  
 
===Source===
 
===Source===

Latest revision as of 20:19, 28 October 2012

RhaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was added to pSB1C3 using Biobrick Assembly Standards.

Source

PCR amplified from Top Ten E. coli using Kapa Hi-Fi polymerase.

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.