Difference between revisions of "Part:BBa K902065:Design"

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===Design Notes===
 
===Design Notes===
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This part was inserted into pSB1C3 using Biobrick Assembly Standards. Note that this promoter is double ended; thus designers must use caution in adding genes to the 5' end of the promoter. The 5' end is intended to express <i>rhaR</i> and <i>rhaS</i> regulatory proteins in native <i>E. coli</i>. The 3' end expresses the <i>rhaBAD</i> operon for rhamnose metabolism is controlled by the regulatory proteins on the opposite side of the promoter.
  
 
===Source===
 
===Source===

Latest revision as of 19:47, 28 October 2012

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was inserted into pSB1C3 using Biobrick Assembly Standards. Note that this promoter is double ended; thus designers must use caution in adding genes to the 5' end of the promoter. The 5' end is intended to express rhaR and rhaS regulatory proteins in native E. coli. The 3' end expresses the rhaBAD operon for rhamnose metabolism is controlled by the regulatory proteins on the opposite side of the promoter.

Source

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.

Jeske, M., & Altenbuchner, J. (2010). The escherichia coli rhamnose promoter rhaP BAD is in pseudomonas putida KT2440 independent of crp--cAMP activation. Applied Microbiology and Biotechnology, 85(6), 1923-1933.