Difference between revisions of "Part:BBa K914018"

 
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The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
 
The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K914018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K914018 SequenceAndFeatures</partinfo>
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===Characterization===
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[[Image:SCquantitative**2.png|thumb|center|800px|'''Figure 4A and 4B:''' The bar graph represent the OD<sub>600</sub> at time = 8h20' (black line Figure 4C) for the different strains at a given kanamycin concentration (black line on Figure 4B). On B, we observe the variations of the OD<sub>600</sub> at different kanamycin concentrations, at the same time.]]
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[[Image:SCquantitative**1.png|thumb|center|800px|'''Figure 4C:''' The variations of the OD<sub>600</sub> is observed in function of the time, for different concentrations of kanamycin (400µg/mL, 100µg/mL, 25µg/mL).]]
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K914018 parameters</partinfo>
 
<partinfo>BBa_K914018 parameters</partinfo>
 
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Revision as of 17:09, 28 October 2012

P1003** Kan resistant gene with 2 Amber Codon

The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Figure 4A and 4B: The bar graph represent the OD600 at time = 8h20' (black line Figure 4C) for the different strains at a given kanamycin concentration (black line on Figure 4B). On B, we observe the variations of the OD600 at different kanamycin concentrations, at the same time.
Figure 4C: The variations of the OD600 is observed in function of the time, for different concentrations of kanamycin (400µg/mL, 100µg/mL, 25µg/mL).



Functional Parameters