Difference between revisions of "Part:BBa K818600:Design"

 
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<partinfo>BBa_K818600 short</partinfo>
 
<partinfo>BBa_K818600 short</partinfo>
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This is a coding construct for B. subtilis to produce AmilGFP pigment in response to spoiled meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.
  
 
<partinfo>BBa_K818600 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K818600 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The sboA regulator is derived from B. subtilis, so this coding construct will work best in B. subtilis. A supporting backbone is needed for this construct to work optimally.
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The sboA regulator is derived from <i>B. subtilis</i>, so this coding construct will work best in <i>B. subtilis</i>. A supporting backbone is needed for this construct to work optimally.
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Please have a look at our [http://2012.igem.org/Team:Groningen/protocol protocol page] to see how we subjected our strains to spoiled meat volatiles.
  
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We used our integration vector BBa_K818000 to clone the construct into <i>Bacillus subtilis</i>.
  
  
 
===Source===
 
===Source===
  
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This part is combined from biobricks.
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This part is combined from biobricks: P<i>sboA</i> (BBa_K818100), amilGFP (BBa_K592010) with RBS (B0034).  
  
 
===References===
 
===References===

Revision as of 03:27, 27 October 2012

AmilGFP with sboA regulator

This is a coding construct for B. subtilis to produce AmilGFP pigment in response to spoiled meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sboA regulator is derived from B. subtilis, so this coding construct will work best in B. subtilis. A supporting backbone is needed for this construct to work optimally.

Please have a look at our [http://2012.igem.org/Team:Groningen/protocol protocol page] to see how we subjected our strains to spoiled meat volatiles.

We used our integration vector BBa_K818000 to clone the construct into Bacillus subtilis.


Source

This part is combined from biobricks: PsboA (BBa_K818100), amilGFP (BBa_K592010) with RBS (B0034).

References