Difference between revisions of "Part:BBa K902054:Design"
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===Source=== | ===Source=== | ||
| − | DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry. | + | DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method. |
===References=== | ===References=== | ||
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21 | Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21 | ||
Latest revision as of 02:32, 27 October 2012
Inducible DszB generator (J04500-DszB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 652
Illegal XhoI site found at 644 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 359
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
See DszB mutations
Source
DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method.
References
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21