Difference between revisions of "Part:BBa K812012"

Revision as of 00:09, 27 October 2012

OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.

This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.

This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.

This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 433
Illegal NotI site found at 287
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1648
Illegal XhoI site found at 356
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 55
Illegal NgoMIV site found at 164
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 79

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K812012 short</partinfo>
-OsTiRI is an ubuquitinase E3 that recognise and trigger the ubiquitination of every AID tagged protein. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
+OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
-This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
+This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.
-This part contains 3 myc domain for protein staining using antiMyc antibodies
+This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.
 This part can be micro-injected using pSC2+ ( <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>) or related plasmid as a backbone.