Difference between revisions of "Part:BBa K902081"

Latest revision as of 23:49, 26 October 2012

4S Desulfurization Pathway Optimization Circuit (PlacI/hpaC/RBS/katGLAA)

This part produces HpaC and KatG-LAA under control of an inducible lacI promoter.

KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH₂ while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.

File-Ucalgary team sulfur 4s enzyme pathway diagram.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 993
Illegal BglII site found at 1928
Illegal BglII site found at 2055
Illegal BamHI site found at 1845
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 509

@@ Line 4: / Line 4: @@
 This part produces HpaC and KatG-LAA under control of an inducible lacI promoter.
-KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
+KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.<br> [[Image:File-Ucalgary team sulfur 4s enzyme pathway diagram.png|centre]]
-</html>[[Image:File-Ucalgary team sulfur 4s enzyme pathway diagram.png|centre]]<html></html>
 <!-- Add more about the biology of this part here