Difference between revisions of "Part:BBa K902079:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902079 short</partinfo>
 
<partinfo>BBa_K902079 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
na
+
DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info
 +
 
 +
<i>dszB</i> and <i>dszC</i> were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
na
+
Assembled from biobrick parts
  
 
===References===
 
===References===
 +
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from <i>Rhodococcus erythropolis</i> KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21

Revision as of 23:43, 26 October 2012

PtetR/dszB/B0034/dszC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 506
    Illegal BamHI site found at 2086
    Illegal XhoI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2236


Design Notes

DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info

dszB and dszC were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info


Source

Assembled from biobrick parts

References

Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21