Difference between revisions of "Part:BBa K902017"
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<html> This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For characterization data please click <a href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">here</a>. </html>
 
<html> This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For characterization data please click <a href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">here</a>. </html>
  
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===Usage and Biology===
 
===Usage and Biology===
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The <i>mgt</i> system is used in the control and metabolism of magnesium in <i>E. coli</i>.  It is important in regulating the transport of these ions into and out of the cell to ensure homeostasis is maintained.  It has been previously characterized that the <i>mgtA</i> transport system contains an upstream promoter and riboswitch sensative to magnesium.  We have cloned and biobricked both of these components and shown that they demonstrate the ability
  
 
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Revision as of 23:41, 26 October 2012

MgtAp-mgtArb-GFP(LVA)

This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For characterization data please click here.

Usage and Biology

The mgt system is used in the control and metabolism of magnesium in E. coli. It is important in regulating the transport of these ions into and out of the cell to ensure homeostasis is maintained. It has been previously characterized that the mgtA transport system contains an upstream promoter and riboswitch sensative to magnesium. We have cloned and biobricked both of these components and shown that they demonstrate the ability

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 974