Difference between revisions of "Part:BBa K902002"
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This part contains the beta-glucuronidase (<i>uidA</i>) enzyme from E. coli under the control of an IPTG inducible promoter. | This part contains the beta-glucuronidase (<i>uidA</i>) enzyme from E. coli under the control of an IPTG inducible promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
The <i>uidA</i> gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay. It is also known as the <i>gusA</i> gene and has numerous homologous in various bacterial and plant species. It has specific activity against glucuronoside linked to a variety of other components. This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically. See our experience page for more information on its characterization. | The <i>uidA</i> gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay. It is also known as the <i>gusA</i> gene and has numerous homologous in various bacterial and plant species. It has specific activity against glucuronoside linked to a variety of other components. This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically. See our experience page for more information on its characterization. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K902002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K902002 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:48, 26 October 2012
Beta-glucuronidase (uidA) inducible generator
This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.
Usage and Biology
The uidA gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay. It is also known as the gusA gene and has numerous homologous in various bacterial and plant species. It has specific activity against glucuronoside linked to a variety of other components. This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically. See our experience page for more information on its characterization.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 732
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]