Difference between revisions of "Part:BBa K902002"

Revision as of 21:48, 26 October 2012

Beta-glucuronidase (uidA) inducible generator

This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K902002 short</partinfo>
-This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.
+This part contains the beta-glucuronidase (<i>uidA</i>) enzyme from E. coli under the control of an IPTG inducible promoter.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The <i>uidA</i> gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay.  It is also known as the <i>gusA</i> gene and has numerous homologous in various bacterial and plant species.  It has specific activity against glucuronoside linked to a variety of other components.  This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically.  See our experience page for more information on its characterization.
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 <span class='h3bb'>Sequence and Features</span>