Difference between revisions of "Part:BBa K902041"

Revision as of 21:13, 26 October 2012

Constitutive AmdA Generator

AmdA coding sequence (K902035) downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034). AmdA is an amidase gene from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 670
Illegal XhoI site found at 162
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 170
Illegal NgoMIV site found at 1375
Illegal NgoMIV site found at 1481
Illegal AgeI site found at 548
Illegal AgeI site found at 732
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 406
Illegal BsaI site found at 947

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 <partinfo>BBa_K902041 short</partinfo>
-<html><i>AmdA</i> coding sequence (<a href="https://parts.igem.org/Part:BBa_K902032">K902035</a>) downstream of a strong TetR repressible promoter (<a href="https://parts.igem.org/Part:BBa_R0040">R0040</a>) and a ribosome binding site (<a href="https://parts.igem.org/Part:BBa_R0040">B0034</a>). <i>AmdA</i> is an amidase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.
+<html><i>AmdA</i> coding sequence (<a href="https://parts.igem.org/Part:BBa_K902035">K902035</a>) downstream of a strong TetR repressible promoter (<a href="https://parts.igem.org/Part:BBa_R0040">R0040</a>) and a ribosome binding site (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>). <i>AmdA</i> is an amidase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.
 </html>[[Image:amidase pathway kilbane calgary12.jpg|center]]