Difference between revisions of "Part:BBa K902041"

 
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<partinfo>BBa_K902041 short</partinfo>
 
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AmdA coding sequence downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034).  
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AmdA coding sequence downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034). <i>AmdA</i> is an amidase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.
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</html>[[Image:amidase pathway kilbane calgary12.jpg|center]]
  
 
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Revision as of 21:08, 26 October 2012

Constitutive AmdA Generator

AmdA coding sequence downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034). AmdA is an amidase gene from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.

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Amidase pathway kilbane calgary12.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 670
    Illegal XhoI site found at 162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1481
    Illegal AgeI site found at 548
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406
    Illegal BsaI site found at 947