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<i>AmdA</i> is a deaminase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid.  
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<i>AmdA</i> is an amidase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid.  
 
</html>[[Image:amidase pathway kilbane calgary12.jpg|center]]
 
</html>[[Image:amidase pathway kilbane calgary12.jpg|center]]
  

Latest revision as of 21:07, 26 October 2012

AmdA Coding Sequence

AmdA is an amidase gene from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid.
Amidase pathway kilbane calgary12.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 590
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 468
    Illegal AgeI site found at 652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal BsaI site found at 867