Difference between revisions of "Part:BBa K902041:Experience"
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<i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below: | <i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below: | ||
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</html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> | </html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> | ||
| − | </html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure | + | </html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine. Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]<html>|}; |
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Revision as of 09:58, 26 October 2012
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