Difference between revisions of "Part:BBa K801076:Design"

(→‎Design Notes)
(→‎Source)
Line 73: Line 73:
  
 
'''Source:'''<br>
 
'''Source:'''<br>
 +
* Preexisting BioBrick BBa_K801070 and BBa_K801071
 
<!--*Commercial system: plasmid name, system name, company name-->
 
<!--*Commercial system: plasmid name, system name, company name-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
Line 83: Line 84:
  
 
'''Organism:'''<br>
 
'''Organism:'''<br>
 +
* Genesequence derived from ''coffea arabica''
 +
* codonoptimized for ''saccharomyces cerevisiae''
 +
* designed for ''saccharomyces cerevisiae''
 +
 
<!--*Genesequence derived from ''citrus limonen''-->
 
<!--*Genesequence derived from ''citrus limonen''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->

Revision as of 10:58, 23 October 2012

Yeast expression cassette for CaXMT1 and CaMXMT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385

Composite part of BBa_K801010, BBa_K801070, BBa_K801012, BBa_K319003, BBa_K801071 and BBa_K801012


Keywords: caffeine, TEF1, expression cassette


Abbreviations:

Design Notes

Related BioBrick: BBa_K801070 BBa_K801071 BBa_K801077

Quality control measures:

  • Test digest has been performed with use of Xba1 and Pst1 (see mainpage)

Backbone:

  • Backbone name: pSB1C3'
  • Resistance: Cp

Protein coding:

  • Protein: CaXMT1-Strep-tag and CaMXMT1-Strep-tag


Enzymatic activity: EC-number [http://enzyme.expasy.org/EC/2.1.1.158 2.1.1.158] EC-number [http://enzyme.expasy.org/EC/2.1.1.160 2.1.1.160]


Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBrick BBa_K801075 is further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • Preexisting BioBrick BBa_K801070 and BBa_K801071


Organism:

  • Genesequence derived from coffea arabica
  • codonoptimized for saccharomyces cerevisiae
  • designed for saccharomyces cerevisiae


References

Literature references:

Database references: