Difference between revisions of "Part:BBa K801074:Design"

Revision as of 10:26, 23 October 2012

CaMXMT1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1433
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 205

Composite part of BBa_K319003, BBa_K801071 and BBa_K801012

Keywords: caffeine, TEF1, expression cassette

Abbreviations:

Design Notes

Related BioBrick: [1]

Quality control measures:

Test digest has been performed with use of Xba1 and Pst1 (see mainpage)

Backbone:

Backbone name: pSB1C3'
Resistance: Cp

Protein coding:

Protein: CaMXMT1-Strep-tag

Enzymatic activity:

EC-number [http://enzyme.expasy.org/EC/2.1.1.160 2.1.1.160]

Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBricks BBa_801073 and BBa_801075 are further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references:

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 '''Related BioBrick:'''
-* [https://parts.igem.org/wiki/index.php?title=Part:BBa_K801071]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801071]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]