Difference between revisions of "Part:BBa K801020:Design"
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'''Related BioBrick:''' | '''Related BioBrick:''' | ||
| − | * Related biobrick[https://parts.igem.org/wiki/index.php?title=Part:BBa_K678001 BBa_K678001]: PalcA, inducible ''Aspergillus nidulans'' promoter | + | * Related biobrick: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678001 BBa_K678001]: PalcA, inducible ''Aspergillus nidulans'' promoter |
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]---> | <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]---> | ||
Latest revision as of 15:51, 22 October 2012
KlADH4 yeast promoter, ethanol inducible
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 753
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 590
The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from Mazzoni et al.,2000.
Extracted from genomic DNA of K. lactis using PCR.
Keywords:
ethanol inducible, KlADH4, yeast, promoter, inducible, K. lactis, S. cerevisiae
Abbreviations:
Design Notes
Related BioBrick:
- Related biobrick: BBa_K678001: PalcA, inducible Aspergillus nidulans promoter
Cloning details:
- Designed in RFC25
- To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis
Quality control measures:
- Test digestion using EcoRI and SpeI
- Part was totally sequenced
Backbone:
- Backbone name: pSB1C3'
- Resistance: Cam
- Copynumber: high
Protein coding:
-
Enzymatic activity:
-
Cytotoxicity:
- none
Safety notes:
- Known and anticipated sefety issues: none
- Known and anticipated security issues: none
Intellectual property:
Corresponding part author/authors:
Source
Source:
- Cloned from genomic DNA of Kluyveromyces lactis strain WSYC115
- Forward Primer:
5'- AGT CAG TCT AGA ACG ACG GTA CAT AAG AAT GAG G - 3' - Reverse Primer:
5'- GGC TAA GGT ACC CAT ATG GTG TGT TGT ATG TTG TGT TGT TGG - 3'
Organism:
- Sequence derived from Kluyveromyces lactis
References
Literature references:
- [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 9872759]
- [2] Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 10724480]