Difference between revisions of "Part:BBa K819005"
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<a href="/Part:BBa_K819005">Summary</a>
 
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<a href="/Part:BBa_K819005:Designing">Design</a>
 
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<a href="/Part:BBa_K819005:Characterization">Characterization</a>
 
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<b>Summary</b>
 
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This part, which is a fusion protein, serves as <b>an ultra sensitive photoreceptor</b> (which can sense light as weak as moonlight) and will induce a <b>light-dependent repression</b> of genes under specific promoters like [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 RecA408 promoter] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 SulA408 promoter].
 
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For the photosensor domain of this fusion protein, we chose VVD protein, the smallest photosensor protein known, which forms a rapidly exchanging dimer when excited by blue light. For the physiological function domain, we chose LexA protein, a bacteria transcription inhibitor, whose DNA binding domain rigorously requires help of the dimerization domain for its DNA binding activity. We fused these two separate domains together to form a fusion protein, which we termed our "Luminesensor". Both DNA binding domain and dimerization domain of this luminesensor was mutated at specific sites for better bio-orthogonality and higher on-off ratio.(to see the <b>design</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Designing here])
 
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We then characterized this luminesensor in different ways and all the results showed that this luminesensor could response to light quite well. (to see the <b>characterization</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Characterization here])
 
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To learn more details about this luminesensor and the related project by Peking 2012 iGEM team, please visit our [http://2012.igem.org/Team:Peking wiki]
 
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Revision as of 04:43, 20 October 2012

Constitutive LuxBrick Generator



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726