Difference between revisions of "Part:BBa J45002:Design"
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*Bases 1-18 of the original ''A. majus'' sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon. | *Bases 1-18 of the original ''A. majus'' sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon. | ||
*A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site. | *A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site. | ||
| + | *The original stope (TGA at bp ___) was deleted and a double TAA stop was inserted | ||
===Source=== | ===Source=== | ||
Revision as of 23:35, 13 June 2006
SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 970
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:
<biblio>
- Dudareva pmid=11051108
</biblio>
- Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
- A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
- The original stope (TGA at bp ___) was deleted and a double TAA stop was inserted
Source
Genbank accession number(s): AF198492
We got this cDNA from N Dudareva at Purdue.