Difference between revisions of "Part:BBa K781005"

Latest revision as of 12:38, 14 October 2012

[R0010][B0034] - LinB Dehalogenase-FliC Insertion Chimera

LinB is a dehalogenase enzyme. It degrades haloalkanes which are toxic pollutants that accumulate in food chains. This part has the LinB incorporated at an insertion site.

Fig. 4 The motility assay for the K781005 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2383
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1676
Illegal AgeI site found at 530
Illegal AgeI site found at 938
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 LinB is a dehalogenase enzyme. It degrades haloalkanes which are toxic pollutants that accumulate in food chains. This part has the LinB incorporated at an insertion site.
-[[Image:linb_mot.png|thumb|500px|left|'''Fig. 4''' The motility assay for the K781005 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct.]]
+[[Image:linb_mot.png|thumb|500px|left|'''Fig. 4''' The motility assay for the K781005 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.]]
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 <!-- Add more about the biology of this part here