Difference between revisions of "Part:BBa K781004"

Latest revision as of 12:38, 14 October 2012

[R0010][B0034] - Rv2579 Dehalogenase-FliC Insertion Chimera

Rv2579 is a dehalogenase enzyme. This construct as incorporated that enzyme as an insertion into the variable domain.

Fig. 4 The motility assay for the K781004 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2389
Illegal XhoI site found at 1691
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1167
Illegal NgoMIV site found at 1432
Illegal AgeI site found at 530
Illegal AgeI site found at 938
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Rv2579 is a dehalogenase enzyme. This construct as incorporated that enzyme as an insertion into the variable domain.
-[[Image:rv2579_edit.png|thumb|500px|left|'''Fig. 4''' The motility assay for the K781004 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct.]]
+[[Image:rv2579_edit.png|thumb|500px|left|'''Fig. 4''' The motility assay for the K781004 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.]]
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 <!-- Add more about the biology of this part here