Difference between revisions of "Part:BBa I721001:Experience"
(→User Reviews) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 17: | Line 16: | ||
Enter the review inofrmation here. | Enter the review inofrmation here. | ||
|}; | |}; | ||
| + | <!-- End of the user review template --> | ||
| + | <!-- DON'T DELETE --><partinfo>BBa_I721001 EndReviews</partinfo> | ||
| + | |||
| + | |||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_I721001 AddReview number</partinfo> | ||
| + | <I>Username</I> | ||
| + | |width='60%' valign='top'| | ||
| + | We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results. | ||
| + | |||
| + | |||
| + | <center>https://static.igem.org/mediawiki/2012/c/c4/Leadgraph.jpg</center> | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_I721001 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_I721001 EndReviews</partinfo> | ||
Revision as of 23:09, 13 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I721001
User Reviews
UNIQfb1eac28603484bb-partinfo-00000000-QINU UNIQfb1eac28603484bb-partinfo-00000001-QINU
|
No review score entered. Username |
We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results.
 UNIQfb1eac28603484bb-partinfo-00000003-QINU |