Difference between revisions of "Part:BBa K814002"
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Figure 1. PCR screening of O-MT from colonies following ligation and transformation into the pUCBB plasmid.
 
Figure 1. PCR screening of O-MT from colonies following ligation and transformation into the pUCBB plasmid.
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Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:54, 13 October 2012

dehydroquinate O-methyltrasferase (O-MT) generator

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. Dehydroquinate O-methyltransferase (O-MT) catalyzes the second step in this pathway, converting dehydroquinate to 4-deoxygadusol.

This part includes a modified constitutive lac promoter (lacPmod), and RBS and the open reading frame of O-MT. This part can be used to express O-MT in E. coli.

Dhqs.png

Figure 1. PCR screening of O-MT from colonies following ligation and transformation into the pUCBB plasmid.

Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 418
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
    Illegal XhoI site found at 988
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 418
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 418
    Illegal AgeI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]