Difference between revisions of "Part:BBa K814005"

 
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This composite part contains protein generators for DHQS, O-MT, ATPG and the shinorine NRPS.  
 
This composite part contains protein generators for DHQS, O-MT, ATPG and the shinorine NRPS.  
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[[Image:Doan.png]]
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Figure 1. The shinorine biosynthetic pathway. 
  
 
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Revision as of 22:40, 13 October 2012

shinorine biosynthetic pathway

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS).

This composite part contains protein generators for DHQS, O-MT, ATPG and the shinorine NRPS.


Doan.png

Figure 1. The shinorine biosynthetic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
    Illegal XbaI site found at 6853
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal NotI site found at 1373
    Illegal NotI site found at 2970
    Illegal NotI site found at 4030
    Illegal NotI site found at 6917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal BglII site found at 134
    Illegal BglII site found at 1587
    Illegal BglII site found at 3184
    Illegal BglII site found at 4244
    Illegal XhoI site found at 1381
    Illegal XhoI site found at 2978
    Illegal XhoI site found at 4038
    Illegal XhoI site found at 6925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
    Illegal XbaI site found at 6853
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
    Illegal XbaI site found at 6853
    Illegal AgeI site found at 3568
  • 1000
    COMPATIBLE WITH RFC[1000]