Difference between revisions of "Part:BBa K814004"

 
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<partinfo>BBa_K814004 short</partinfo>
 
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This composite part contains protein generators for DHQS, O-MT and ATPG.  E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media.  4-deoxygadusol is also detected in HPLC analysis.
 
This composite part contains protein generators for DHQS, O-MT and ATPG.  E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media.  4-deoxygadusol is also detected in HPLC analysis.
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[[Image:Hplcuv.png]]
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Figure 1. HPLC data of a mycosporine glycine biosynthesis pathway.  The DA construct is shown as a negative control, lacking mycosporine glycine and 4-deoxygadusol production.  Clear peaks for these compounds are observed in the DOA sample.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:33, 13 October 2012

mycosporine-glycine biosynthetic pathway

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG).

This composite part contains protein generators for DHQS, O-MT and ATPG. E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media. 4-deoxygadusol is also detected in HPLC analysis.

Hplcuv.png

Figure 1. HPLC data of a mycosporine glycine biosynthesis pathway. The DA construct is shown as a negative control, lacking mycosporine glycine and 4-deoxygadusol production. Clear peaks for these compounds are observed in the DOA sample.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal NotI site found at 1373
    Illegal NotI site found at 2970
    Illegal NotI site found at 4030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal BglII site found at 134
    Illegal BglII site found at 1587
    Illegal BglII site found at 3184
    Illegal XhoI site found at 1381
    Illegal XhoI site found at 2978
    Illegal XhoI site found at 4038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 3468
    Illegal AgeI site found at 3568
  • 1000
    COMPATIBLE WITH RFC[1000]